Journal: bioRxiv

Article Title: An Enzyme-Linked Immunosorbent Assay (ELISA)-based Activity Assay for AMP-Activated Protein Kinase (AMPK)

doi: 10.1101/2024.08.20.608877

Figure Lengend Snippet: Compound C (5 μM; an AMPK inhibitor), compound 991 (250 nM; an AMPK activator), PF-739 (250 nM; an AMPK activator) or DMSO control were added into the kinase reaction (40 min reaction time) in the presence or absence of AMP. OD values from the ELISA following the kinase reactions were measured and compared, n = 4. For each treatment condition, the OD value from 0 min reaction was determined and considered as blank, and was subtracted from the OD value of the experimental condition. **** p < 0.0001 when comparing to control condition with the same AMP availability in the reaction using Student’s t-test. $ p < 0.05, $$$ p < 0.001, $$$$ p < 0.0001 when comparing to the condition with the same modulator treatment but with the presence of AMP using Student’s t-test. Data are mean ± s.e.m.

Article Snippet: AMPK inhibitor, compound C (cat# AOB2281), and AMPK activators, compound 991 (cat# AOB8150) and PF-739 (cat# AOB33584), were purchased from AOBIOUS (Gloucester, MA, USA).

Techniques: Control, Enzyme-linked Immunosorbent Assay